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KMID : 0829220010250020115
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Korean Journal of Oral and Maxillofacial Pathology
2001 Volume.25 No. 2 p.115 ~ p.126
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S-100α, S-100β, GFAP, NSE and Vimentin expression in Salivary Gland Tumors : Immunohistochemical Study
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Abstract
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A variety of histogenetic concepts for salivary gland tumors have been evolved, but controversial now. It is generally accepted that specific reserve cell or basal cells of the excretory and intercalated ducts or both are resposible for neoplastic transformation. The purpose of this study was to examine the positive tumor cell distribution by various Abs according to the histopathologic structure of Pleomorphic adenoma(PA), Adenoid cystic carcinoma (ACC), and Mucoepidermoid carcinoma(MFCa), and to understand the role of modified or neoplastic myoepithelial into the tumorogenesis of salivary gland tumors. We used 4 cases of PA, 6 cases of ACC(Cribriform type(3 cases), Tubular type (2 cases), and Solid type(1 cases), and MFCa(3 cases). For immunohistochemical staining 4㎛ deparaffinized paraffin sections were incubated with primary antibodies(S-100α, S-100β, GFAP, NSE and Vimentin), incubated with LSAB method, treated with DAB, and examined with light microscopy according to 5 degree of stainability (-,+/-,+,++,+++). The obtained results were as follows.
1. In Normal human salivary gland, S-100α was positive in serous acinar cells , and ductal cells, while S-100β and vimentin was mild positivity in ductal cells.
2. In PA, S-100α and S-100β was heterogenous positive in luminal cells, outer layer cells, modified myoepithelial(MM) cells. S-100α and S-100β showed mild positivity in tumor cells of myxoid area, while S-100α, S-100β, GFAP, and NSE was expressed mild to moderately in tumor cells of hyalinized area.
3. In ACC, S-100α was positive in luminal cells and outer layer cells of cribriform type , and luminal cells of tubular type, while S-100β was in luminal cell surface of tubular type. Vimentin was in outer layer cells of cribriform and tubular type, and S-100α showed focal positivity in solid type.
4. In MECa, S-100α showed restrictive positivity in mucous, and intermediate cells, while intermediate and squamous tumor cells showed focal vimentin expression.
From the above results, it was suggested that modified myoepithalial cells could paly a role into tumorogenesis of PA, and be partially associated with cribriform and tubulo-ductual structures of ACC, but not with solid structure, and could not play a major role into tumorgenesis of Mucoepidermoid carcinoma.
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KEYWORD
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